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Image Search Results
Journal: The American Journal of Pathology
Article Title: Expression and Potential Role of Fas-Associated Phosphatase-1 in Ovarian Cancer
doi: 10.1016/s0002-9440(10)64084-9
Figure Lengend Snippet: Figure 2. Comparison of FAP-1 protein levels in ovarian cancer cell lines. Lysates from cell lines (100 g total protein) were subjected to SDS-PAGE/ immunoblot assay using rabbit polyclonal no. 1730. Jurkat and HEK 293 cells served as negative and positive controls, respectively. A nonspecific band is also observed (asterisk), serving as a loading control. Densitometrical anal- ysis was performed using a Multi Image light cabinet and the ChemiImager software version 4000 yielding values based on integrated density (#). Data were arbitrarily normalized relative to FAP-1 levels of HEK 293 cells that were included on all blots as an internal control.
Article Snippet: To quantify immunoblotting results, films were analyzed densitometrically using a
Techniques: Comparison, SDS Page, Western Blot, Control, Software